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DMBA 매식과 방사선 조사로 유도된 백서 타액선 종양에서 H-ras 암유전자의 활성화
ACTIVATION OF H-RAS ONCOGENE IN RAT SALIVARY GLAND TUMORS INDUCED BY DMBA AND IRRADIATION

치과방사선 1998년 28권 1호 p.245 ~ 260

소속 상세정보

허기순/Key-Soon Hu 최종환/최순철/박태원/유동수/Jong-Whan Choi/Soon-Chul Choi/Tae-Won Park/Dong-Soo You

KMID : 0378219980280010245

Abstract

화학 발암제인 DMBA를 백서 타액선에 매식하여 종양을 유발시킨 실험군과 DMBA 매식
과 방사선 조사로 타액선 종양을 유발시킨 실험군에서 각각 DNA를 분리하여 transfection
분석과 2단계 PCR-RFLP을 시행하였을 때 다음과 같은 결론을 얻었다.
1. 두 실험군에서 종양 DNA를 각각 분리하여 NIH 3T3 세포에 transfection시켰을 때 형
질전환 국한점이 발생하였다.
2. Transfection 분석을 시행하였을 때 형질전환 발생율은 DMBA를 매식한 실험군에서
DNA g당 0.01-0.02 foci였고, DMBA 매식과 방사선 조사를 한 실험군에서 0.01-0.03 foci였
다.
3. 2단계 PCR-RFLP을 이용하여 H-ras유전자의 점 돌연변이 발생율을 조사하였을 때
DMBA를 매식한 실험군에서 13.9%(5/36)로 나타났고, DMBA 매식과 방사선 조사를 병행한
실험군에서는 15.4%(6/39)로 나타났다.
4. DMBA를 매식한 실험군에서 H-ras 유전자의 12번째 codon의 점 돌연변이가
5.6%(2/36)로 나타났고 61번째 codon에서는 8.3%(3/36)로 나타났다.
5. DMBA 매식과 방사선 조사를 시행한 실험군에서 H-ras유전자의 12번째 codon의 점
돌연변이가 7.7%(3/39)로 나타났고 61번째 codon에서도 7.7%(3/39)로 나타났다.
#초록#
Cellular transforming genes have been identified in a number of different tumor cell
lines and tumor types. A significant number of these oncogenes belong to the ruts gene
family. The rats gene family consists of three closely related genes : H-ras, K-ras and
N-ras which code for a related 21 kDa protein. Mutations in codon 12, 13 and 61 of one
of the three rats genes convert these genes into acute oncogenes. The presence of
H-ras gene mutations has important prognostic implications in various tumors.
Each genomic DNA was isolated from tumors induced by implantation with DMBA, or
by treatment with DMBA-implantation/irradiation. When genome DNA was transfected
into NIH 373 cells and investigated by two-step PCR-RFLP, the following results were
concluded :
1. Transformation foci developed in two groups when the genome DNA of two
experimental groups were transfected into NIH 3T3 cells.
2. Transformation efficiency was 0.01-0.02 foci/㎍DNA in the experimental group with
the DMBA-implantation, 0.01-0.03 foci/㎍DNA in the experimental group with the
DMBA -implantation/irradiation according to results of transfection assay.
3. When the point mutation of H-ras gene was investigated by a two-step
PCR-RFLP, there was 13.9% (5/36) in the experimental group with the DMBA
implantation, 15.4%(6/39) in the expermetal group with the
DMBA-implantation/irradiation.
4. The point mutation in codon 12 and 61 of H-ras was 5.6%(2/36) and 8.3%(3/36) in
the experimental group with the DMBA implantation.
5. The point mutation in codon 12 an4 61 of H-ras gene was 7.7%(3/39) in the
experimental group with the DMBA-implantation/irradiation.